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KMID : 0359320020420020171
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Korean Journal of Veterinary Research
2002 Volume.42 No. 2 p.171 ~ p.181
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Matrix solid phase dispersion isolation and high performance liquid chromatographic determination of five benzimidazole anthelmintics in bovine muscle, liver and omasum
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Kim Chung-Hui
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Abstract
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Simultaneous multiresidue analysis using liquid chromatography determination for five benzimidazole anthelmintics(thiabendazole, oxibendazole, albendazole, mebendazole and fenbendazole) in bovine muscle, liver and omasum has been described. Blank or benzimidazole-fortified samples(0.5g) were blended with bulk C_18(40㎛, 18% load, endcapped, 2g). A column made from the resultant C_18/animal tissue matrix was first washed with hexane(8㎖), following which the benzimidazoles were eluted with acetonitrile(8㎖). Analytes of extracted sample were determined by liquid chromatography with UV detector at 290nm. Correlation coefficients of standard curves for individual benzimidazole isolated from fortified samples, using internal standardization, were linear(0.991±0.007 to 0.996±0.005) with average relative percentage recoveries from 62.1±3.8(%) to 92.3±7.5(%) for the concentration range(0.2∼6.4㎍/g), respectively. Recoveries rates of TBZ, MBZ in liver, OBZ, MBZ in muscle and TBZ, MBZ in omasium from fortified benzimidazole were 92.%, 87.3%, 74.5%, 82.7%, 75.2%, and 83.5% at condition Ⅱ, respectively. Condition Ⅱ showed higher recoveries rates than codition Ⅰ.
These results indicated that the matrix solid phase dispersion(MSPD) methodology is acceptable forthe determination of 5 benzimidazole anthelmintics and may also suitable for other matrixes of food animal origin.
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